UJI AKTIVITAS BAKTERI PENAMBAT NITROGEN DAN PENGHASIL IAA DARI RIZOSFER TANAMAN KEDELAI ( Glycine max L.)

saida saida, Puspitasari Puspitasari, Aminah Aminah

Abstract


This study aims to examine the activities of nitrogen-fixing bacteria and Indole Acetic Acid (IAA)-producing bacteria from the soybean plant rhizosphere. This research was conducted at the Laboratory of Plant Pests and Diseases, Faculty of Agriculture, Universitas Muslim Indonesia (UMI), Makassar. Analysis of the sample N fastening test was carried out at the Laboratory of Soil and Conservation, Faculty of Agriculture, UMI, Makassar. Analysis of the IAA test sample was carried out at the Laboratory of Plant Pests and Diseases, Faculty of Agriculture, Hasanuddin University, Makassar, from May to July 2021. This study used a Completely Randomized Design (CRD) method. The isolates tested were isolates obtained from soybean roots, namely isolate 2.2, isolate 2.3 and isolate 3.2. Of the three isolates, each was replicated three times, so that there were nine replications. The obtained data was then analyzed using the Anova test.The results showed that isolate 3.2 had a higher nitrogen fixation ability, which was 20.31% compared to isolate 2.2 and isolate 2.3. The density level of isolate 2,3 tended to be higher, with an absorbance of 0.43 and the ability to produce IAA at 6.61 ppm.

Keywords


Plant Rhizosphere; IAA; Nitrogen; Soybean Plants

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References


Astriani, M. 2015. Seleksi Bakteri Penghasil Indole-3-Acetic Acid (IAA) dan Pengujian Pada Bibit Kelapa Sawit (Elais guneensus Jacq.).

Thesis. Sekolah Pascasarjana, Institut Pertanian Bogor. Bogor.

Bric, J.M., Richard, M.B., & Sara, E.S. 1991. Rapid In Situ Assay for Indoleacetic Acid Production by Bacteria Immobilized on a Nitrocellulose Membrane. Appli Environ Microbiol. 57: 535-538.

Firrani, M. 2011. Isolasi dan Uji Kemampuan Bakteri Endofit Diazotrof yang Memfiksasi Nitrogen Bebas pada Akar Kelapa Sawit (Elaeis guineensis Jaqc.). Skripsi. Medan. Universitas Sumatera Utara.

Hindersah, R., & T. Simarmata. 2004. Potensi Rhizobacteri Azotobacter Dalam Meningkatkan Kesehatan Tanah. J Natura Indones. 5:127-133.

Lestari P, DN Susilowati and EI Riyanti. 2007. Pengaruh hormon asam indol asetat yang dihasilkan Azospirillum sp. terhadap perkembangan akar padi. Jurnal AgroBio-gen 2, 66-72.

Novriani. 2011. Pernanan Rhizobium dalam Meningkatkan Ketersediaan Nitrogen bagi Tanaman Kedelai. Agronobis. Vol. 3, No. 5.

Pamungkas, F. T., Sri, D1 & Raharjo, B. 2009. Pengaruh Konsentrasi dan Lama Perendaman dalam Supernatan Kultur Bacillus sp.2ducc-br-kl.3 Terhadap Pertumbuhan Stek Horisontal Batang Jarak Pagar (Jatropha curcas L.). Artikel Penelitian. Universitas Diponegoro.

Seniati, Marbiah dan Irham A. 2019. Pengukuran Kepadatan Bakteri Vibrio Harveyi secara Cepat dengan Menggunakan Spectrofotometer. Jurnal Agrokompleks 19 (2) : 12-19.

Sukmadi, R. B. 2012. Aktivitas Fitohormon Indole-3-Acetic-Acid (IAA) dari Beberapa Isolat Bakteri Rhizosfer dan Endofit. Jurnal Sains dan Teknologi Indonesia, 14 (3), 221-227.

Widi, I.K. 2012. Perhitungan Bakteri. Program Studi Budidaya Perairan Fakultas Pertanian Universitas Lampung.




DOI: https://doi.org/10.33096/agrotek.v6i1.176

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AGROTEK: Jurnal Ilmiah Ilmu Pertanian
ISSN 2581-3021
Published by Program Studi Agroteknologi Fakultas Pertanian Universitas Muslim Indonesia

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