KAJIAN PATOGENISITAS Beauveria bassiana dan Metarhizium sp TERHADAP LARVA ULAT GRAYAK (Spodoptera frugiperda) PADA TANAMAN JAGUNG

Yasir Harun, Ayu Kartini Parawansa, Abdul Haris

Abstract


Spodoptera frugiperda is an important pest on maize. The purpose of this research were to determine the pathogenicity of Beauveria bassiana and Metharizium sp in Fall armyworm (S. frugiperda) larvae, to determine the effective concentrations of B. bassiana and Metarhizium sp. to control  Fall armyworm (S. frugiperda), and to determine the time span of B. bassiana and Metarhizium sp infection which is effective for controlling Fall armyworm (S. frugiperda). This research was conducted at the Pesticide and Natural Materials Laboratory, Faculty of Agriculture, Universitas Muslim Indonesia, Makassar, UPTD Balai Proteksi of South Sulawesi and Corn Planting in Galung Village, Barru District. Barru Regency. The research start on April to October 2020. The research used a Fully Randomized Design (FRD) with 2 factors and three repetition. The first factor was the type of fungus (Metarhizium sp and B. bassiana) and the second factor was the density of conidia (10⁶, 10⁷, 10⁸ and 10⁹ conidia/ml). The results showed that the type of fungus and the density of conidia were significantly different for all observed variables. The pathogenicity of the fungus B. bassiana was better than Metarhizium sp and the density of conidia (concentrations) of both B. bassiana and Metarhizium sp. were significantly different from those of S. frugiperda larvae, but the density of conidia (concentration) 10⁹ was faster infected and killed S. frugiperda larvae. The Symptoms of entomopathogenic fungus attack on S. frugiperda larvae are sluggish movement, turning pale in color, decreased appetite, gradually silence, and death. The dead larvae are hardened and slightly blackened.


Keywords


Pathogenicity; Beauveria bassiana; Metarhizium; Spodoptera frugiperda; Corn

Full Text:

PDF

References


Achmad.T & J. Tandiabang, 2001. Dinamika Populasi Hama Utama Tanaman Jagung Pada Pola Tanam Berbasis Jagung. Balai Penelitian Tanaman Serealia. Maros Sulawesi Selatan.

Ahsol Hasyim, Wiwin Setiawati , Abdi Hudayya dan Luthfy, 2016.

Sinergisme Jamur Entomopatogen Metarhizium anisopliae Dengan Insektisida Kimia untuk Meningkatkan Mortalitas Ulat Bawang

Spodoptera exigua. Balai Penelitian Tanaman Sayuran. Lembang. Jawa Barat

Arianti, M.P.D., F.X. Susilo, & Indriyati. 2000. Daya tular dan keterpautan kepadatan inokulum cendawan Metarhizium anisopliae pada kutudaun kedelai (Aphis glycines). J. Pen. Pengb. Wil. Lahan Kering 22 (1): 21-27.

Baehaki, S.E. & Noviyanti. 1993. Pengaruh umur biakan Metarhizium anisopliae strain lokal Sukamandi terhadap perkembangan wereng coklat. Prosiding Makalah Simposium Patologi Serangga I. Yogyakarta, 12-13 Oktober 1993. pp. 113-124.

BPS. 2018. Badan Pusat Statistik. Jakarta. Indonesia

Busvine, J. A. R., 1971. Techniques for testing insecticides. Commonwealth Agricultural Bureau. London. 336 p.

Dewi, M., F.X. Susilo, & A.M. Hariri. 1998. Daya infeksi, efisiensi penularan, dan periode letal penyakit muskardin hijau (Metarhizium anisopliae) pada wereng batang coklat padi (Nilaparvata lugens). Jurnal Penelitian Pertanian 9 (9): 156-166.

Erika Paola et al. 2018. Metarhizium rileyi biopesticide to control Spodoptera frugiperda: Stability and insecticidal activity under glasshouse conditions. Fungal Biology Volume 122, Issue 11, November 2018, Pages 1069-1076

Ferron P. 1985. Pest control by fungi Beauveria and Metarhizium. In: Burgers HD, editor. Microbial of Pest and Plant Dieseses 1970-1980, 465-482. London: Academic Press Inc.

Freimoser F M., Screen S., Bagga S., Hu G & St Leger R J. 2003. Expressed sequence tag (EST) analysis of two subspecies of Metarhizium anisopliae reveals a plethora of secreted proteins with potential activity in insect hosts. Microbiol. 239-247.

Hadioetomo, R. S. 1993. Mikrobiologi Dasar dalam Praktek : Teknik dan Prosedur Dasar Laboratorium. PT Gramedia Pustaka Utama. Jakarta. 163 hal.

Hosang, M. L., A. A. Lolong, D. Michellia dan D. Sitepu, 1989, Pengendalian Hayati Oryctes rhinoceros L. Dengan Baculovirus oryctes dan Metarhizium anisopliae. Balai Penelitian Kelapa. Manado. Balai Penelitian Tanaman Rempah dan Obat. Bogor.

Husein, Umar. (2003). Metode Riset Perilaku Konsumen Jasa. Jakarta : Ghalia Indonesia

Kaur S., Harminder P K., Kirandeep K & Amarjeet K. 2011. Effect of different concentrations of Beauveria bassiana on development and reproductive potential of Spodoptera litura (Fabricius). J. Biopest. 4(2):161-168.

Kementan. 2019. Kementerian Pertanian. Jakarta. Indonesia

Kherb W A A. 2014. Virulence Bio-Assay Efficiency of Beauveria bassiana and Metarhizium anisopliae for the biological control of Spodoptera exigua Hubner (Lepidoptera: Noctuidae) eggs and the 1st instar larvae. Aust. J. Basic & Appl. Sci. 8(3): 313-323.

Luz, C., M.S. Tigano, I.G. Silva, C.M.T. Cordeiro, and S.M. Aljanabi. 1998. Selection of Beauveria bassiana and Metarhizium anisopliae isolates to control Triatoma infestans. Memorias do Instituto Oswaldo Cruz 93: 839−846

Mahr S. 2003. The Entomopathogen Beauveria bassiana. University of Winconsin, Madison.

Nazar, A., Elna, K., dan Jekvi, H., 2005. Pengaruh Tingkat Konsentrasi Spora Jamur Nomuraea rileyi Terhadap Penggerek Batang Gula. Pusat Penelitian Pengembangan Tanaman Industri. Lampung.

Nugroho, A.N., F.X. Susilo, & Indriyati. 1999. Potensi kontak antara inokulum dan inang menentukan efisiensi penularan penyakit muskardin hijau pada kepik hijau kedelai. J. Pen. Pengb. Wil. Lahan Kering. 24 : 28-36.

Nurnina Nonci. 2019. Pengenalan Fall Armyworm (Spodoptera frugiperda J.E. Smith) Hama Baru pada Tanaman Jagung di Indonesia, Balai Penelitian Tanaman Serealia, Maros.

Prayogo Y, 2005. Potensi, Kendala dan Upaya Mempertahankan Keefektifan Cendawan Entomopatogen untuk Mengendalikan Hama Tanaman Pangan. Buletin Palawija, 10: 53-65.

Prayogo, Y. 2006. Upaya mempertahankan keefektifan cendawan entomopatogen untuk mengendalikan hama tanaman pangan. Jurnal Litbang Pertanian 25 (2): 47-54.

Prayogo Y & Tengkano W. 2004. Pengaruh konsentrasi dan frekuensi aplikasi Metarhizium anisopliae isolat kendalpayak terhadap tingkat kematian Spodoptera litura. Sainteks. Jurnal Ilmiah Ilmu-Ilmu Pertanian 3 (10) : 209-216.

Rahayu dan Umrah. 2012. Uji kemampuan formula Beauveria bassiana balsamo bentuk sediaan tablet untuk mengendalikan Penggerek Buah Kakao (Conopomorpha cramerella Snellen sp). Biocelebes, Vol. 6 No. 1 Juni 2012, hlm. 31-39.

http://jurnal.untad.ac.id/jurnal. Biocelebs.

Riyanto, Santoso. 1991. Cendawan Beauveria bassiana dan Cara Pengendalian Guna Pengendalian Hama Bubuk Kopi. Jakarta: Direktorat Bina Perlindungan Tanaman Perkebunan.

Romero, M., M. Estrada, R.F. Castaneda, and M. Lujan. 1997. Morphological and pathogenical characterization of Metarhizium anisopliae (Metsch.) Sorokin. Cane Sugar 15(1): 17−25.

Rosdah Thalib. 2013. Patogenisitas Isolat Beauveria Bassiana Dan Metarhizium Anisopliae Asal Tanah Lebak Dan Pasang Surut Sumatera Selatan Untuk Agens Hayati Scirpophaga Incertulas. Jurusan Hama dan Penyakit Tumbuhan, Faperta, Universitas Sriwijaya, Indralaya.

Rusli Rusdi. 2008. Perbanyakan Beauveria bassiana Pada Limbah Organik, Formulasi Dan Uji Efektivitasnya Sebagai Bioinsektisida Untuk Pengendalian Hama Spodoptera Exigua Hubner (Lepidoptera: Noctuidae) Jurusan Hama dan Penyakit Tumbuhan, Faperta, Universitas Andalas, Limau

S.P Wraight et all. 2010. Comparative virulence of Beauveria bassiana isolates against lepidopteran pests of vegetable crops Journal of Invertebrate Pathology Volume 103, Issue 3, March 2010, Pages 186-19

Sugiyono. 2011. Metode Penelitian Kuantitatif, Kualitatif dan R&D. Bandung: Afabeta

Sugiyono. 2013. Metode Penelitian Pendidikan Pendekatan Kuantitatif, Kualitatif, dan R&D. Bandung: Alfabeta.

Sukamto S dan K Yuliantoro. 2006. Pengaruh suhu penyimpanan terhadap viabilitas Beauveria bassiana (bals.) Vuill. dalam beberapa pembawa. Pelita Perkebunan 22 (1), 40-56

Surtikanti, Yasin M, 2002. Tingkat Mortalitas Ulat Grayak (Spodoptera litura) Aibat Pencelupan Suspensi Beberapa Isolat Beauveria bassiana. Makalah Utama. Disampaikan pada Prosiding Seminar Ilmiah dan Pertemuan Tahunan PEI, PFI dan HPTI XV Sul-Sel, Maros 29 Oktober 2002.

Swain, D.K., S. Herath, A. Pathirane and B.N. Mittra. 2005. Rainfed lowland and flood prone rice: A critical review on ecology and management technology improving the productivity in Asia. Role of Water Sciences in Transboundary River Basin Management. Thailand.

Tantawizal. 2015. Potensi Cendawan Entomopatogen Beauveria bassiana (BALSAMO) Vuillemin untuk Mengendalikan Hama Boleng Cylas formicarius f. Pada Tanaman Ubi Jalar. Balai Penelitian Tanaman Aneka Kacang dan Umbi. Malang.

Wang, C. and J.E. Powell. 2001. Isolation and evaluation of Metarhizium anisopliae for control of Coptotermes formosanus and Reticulitermes flavipes. An Entomol Odyssey of ESA.

Zainal et al. 2015. “Identifikasi Areal Pengembangan Baru Tanaman Jagung Mendukung Upaya Khusus (Upsus) Peningkatan Produksi Jagung Di Sulawesi Tenggara. Kendari. Balai Pengkajian Teknologi Pertanian Sulawesi Tenggara.




DOI: https://doi.org/10.33096/agrotek.v6i2.238

Refbacks

  • There are currently no refbacks.


Copyright (c) 2022 Yasir Harun, Ayu Kartini Parawansa, Abdul Haris

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

___________________________________________________________
AGROTEK: Jurnal Ilmiah Ilmu Pertanian
ISSN 2581-3021
Published by Program Studi Agroteknologi Fakultas Pertanian Universitas Muslim Indonesia

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0