MULTIPLIKASI TUNAS TALAS JEPANG (Colocasia esculenta (L.) Schott var. Antiquorum) DALAM BERBAGAI KONSENTRASI EKSTRAK RAGI DAN EKSTRAK BIJI JAGUNG SECARA IN VITRO

Abdullah Abdullah, Dewi Zahrah, Sudirman Numba

Abstract


This research aimed to study the effect of various concentrations of yeast extract and corn seed extract in MS culture media on shoot Multiplication of Japanese taro. This research was conducted at the Bio-Science and Plant Reproductive Biotechnology Laboratory, Hasanuddin University (UNHAS), Makassar. The study used a completely randomised design with two treatment factors and seven replications. The first factor was yeast extract: 0%, 6%, 8%, and 10%. The second factor is corn seed extract: 0%, 5%, 10%, and 15%. The data were analysed statistically using ANOVA and LSD 5%. The results showed that the corn seed extract addition had affected no significantly. The treatment of yeast extract significantly affected the time of shoot regeneration, number of shoots, shoot height, and number of roots. Shoot. The concentration of yeast extract of 6% was the best for the multiplication of Japanese taro shoots. Adding 10% corn seed extract and 6% yeast extract was the best for multiplying Japanese taro shoots in vitro.

Keywords


Yeast Extract; Corn Seeds Extract; In Vitro; Multiplication; Japanese Taro

References


Dasmika S, R.S Wulandari dan H Darwati. 2015. Penambahan Ragi dan Ekstrak Biji Jagung Terhadap Pertumbuhan Tunas Manggis Secara In Vitro. Jurnal Hutan Lestari.3(1):35-42.

Fukomoto, J., T. Yamamoto, D. Tsuru and K. Tchikawa.1957. Effect of nitrogen source. Proceedings of the international symposium on enzyme chemistry. Tokyo and Kyoto, Pergamon Press. Los Angeles:479 - 482.

Gansau J. A., Roslina J., dan Spiridrin S. J. 2015. Effect Of Yeast Extract And Coconut Water On Protocorm Proliferation And Growth Development Of Dimorphorchis Rossii. Acta Biologica Malaysiana. 4(2)59-63.

George EF, Hall MA, De Klerk GJ. 2008. Plant Propagation by Tissue Culture 3rd Ed. Vol 1. The Background. Springer.Netherland.

Herawati D., Murkarlina dan Z. Zakiah. 2021. Multiplikasi Anggrek Dendrobium sp. dengan Penambahan Ekstrak Jagung (Zea mays) dan Napthalaena Acetis Acid (NAA) secara In Vitro.6(1):38-47.

Karjadi, A. K. 2016. Kultur Jaringan dan Mikropropagasi Tanaman Kentang (Solanum Tuberosum L.). Balai Penelitian Tanaman Sayuran.8:1-10.

Kasi, P.D., S. Cambaba, dan W. Sanggola, 2021. Aplikasi ekstrak jagung dan air kelapa sebagai zat pengatur tumbuh alami pada pertumbuhan awal bibit apel. Jurnal Pertanian Berkelanjutan, 9(3): 195-201.

Kementerian Perdagangan Republik Indonesia. 2020. Umbi-umbian HS 0714.Osaka: Indonesian Trade Promotion Center.

Kementerian Perdagangan Republik Indonesia.2013. Market Brief: Ubi kayu, Ubi jalar dan Talas. Tokyo: Market Brief Atdag Tokyo.

[LIPI] Lembaga Ilmu Pengetahuan Indonesia . 2010. Talas Bisa Jadi Bahan Pangan Alternatif.

Louw, A.E., H. Kesaulya, dan I. J. Lawalata, 2017. Perbanyakan Mikro Colocasia esculenta (L.) Shott var. antiquorum melalui Penggunaan IAA: J. Budidaya Pertanian. 14(1):28-34.

Lubis, Alfindra N W. 2021. Pengaruh Ekstrak Ragi dalam Menginduksi Tunas Krisan (Chrysanthemum indicum L.) pada Beberapa Sumber Eksplan Secara In Vitro.[Sripsi].Padang:Fakultas Pertanian Universitas Andalas.

Pagalla DV, Andi IL, Masniawati, 2015. Respon Pertumbuhan Propagul Pisang Ambon Hijau Musa Acuminata Colla Pada Beberapa Konsentrasi Ekstrak Jagung Muda Secara In Vitro. [Skripsi]. Makassar. Fakultas Pertanian. Universitas Hasanuddin, Makassar.

Pinto, P.S., I.K. Prasetyo, dan J. Rahaju. 2012. Optimasi pertumbuhan bibit apel (Malus sylvestris Mill.) dengan menggunakan cytokinin alami. Primordia,8(2): 38–52.

Pudjiatmoko, 2008. Talas Jepang Satoimo. http://atanitokyo.blogspot.com/2008/04/talas-jepang-satoimo.html. Diakses 15 Agustus 2023.

Sridhar TM, Aswath CR. 2014a. Influence of Additives on enhanced in vitro Shoot Multiplication of Stevia rebaudiana (Bert.)—An Important Anti Diabetic Medicinal Plant. Am. J. of Plant Scie.,5:192-199.

Temesgen, M dan N. Ratta. 2015. Nutritional potential, Health and Food Security Benefits of Taro Colocasia esculenta (L.): A Review. The Open Food Science Journal 36:23-30.

Ulfa F, 2014. Peran Senyawa Bioaktif Tanaman Sebagai Zat Pengatur Tumbuh Dalam Memacu Produksi Umbi Mini Kentang Solanum tuberosum L. Pada Sistem Budidaya Aeroponik [Disertasi]. Makassar. Program Studi Ilmu Pertanian Pasca Sarjana. Universitas Hasanuddin, Makassar.

Wati RS, Isda MN, Fatonah S, 2015. Induksi Tunas dari Eksplan Bonggol Pisang Udang (Musa acuminata Colla) Secara In Vitro Pada Media MS dengan Penambahan BAP dan Kinetin. Repository FMIPA. Pekan Baru.

Universitas Bima Widya Pekanbaru. Hal 1-6.

Widiastoety, D. dan Kartikanigrum. 2003. Pe

manfaatan Ekstrak Ragi dalam Kultur In Vitro Planlet Media Anggrek. Cianjur J. Hort. 2:60-66.

Zulkarnain, H. 2009. Kultur Jaringan. Jakarta:Bumi Aksara.




DOI: https://doi.org/10.33096/agrotek.v7i2.354

Refbacks

  • There are currently no refbacks.


Copyright (c) 2023 Abdullah Abdullah, Dewi Zahrah, Sudirman Numba

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

___________________________________________________________
AGROTEK: Jurnal Ilmiah Ilmu Pertanian
ISSN 2581-3021
Published by Program Studi Agroteknologi Fakultas Pertanian Universitas Muslim Indonesia

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0