Effects of Trichoderma Doses and Coconut Water as Natural Plant Growth Regulator on the Vegetative Growth of Lime Cuttings

Andi Muh Alfian Damasyhar, Netty Netty, Hidrawati Amdo Ala

Abstract


Vegetative propagation through cuttings is relatively simple; however, lime cuttings (Citrus aurantiifolia) often lack roots capable of supporting upright growth. Natural plant growth regulators such as coconut water (auxins/cytokinins) stimulate rooting, while Trichoderma sp. enhances root development, nutrient absorption, and resistance to pathogens. This study evaluates the effect of Trichoderma dosage and coconut water concentration on the growth of lime cuttings. The experiment used a 3 × 4 factorial randomized block design: Trichoderma (0, 25, 50 g plant⁻¹) and coconut water (100%, 15%, 25%, 35% v/v) in a Green House (April–July 2025). Trichoderma and coconut water significantly affected the time to bud emergence and number of leaves, but their interaction was not significant for these traits; neither had an effect on the number of shoots. The fastest bud emergence occurred with 50 g plant⁻¹ Trichoderma (7.9 days) and 100% coconut water (7.89 days), while 15% coconut water delayed it (10.67 days). Trichoderma 50 g plant⁻¹ increased shoot length (10.9 cm) and leaf number (9.1) compared to the control (9.0 cm; 6.9 leaves). Coconut water 100% maximized shoot length (11.0 cm) and leaves (9.3) compared to 15% (9.0 cm; 6.7 leaves). Success reached 100% with combinations of Trichoderma 25 or 50 g and 100% coconut water. Application of Trichoderma 50 g plant⁻¹ and undiluted coconut water effectively accelerated early shoot growth and improved the performance of lime cuttings.

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References


Andrzejak, R., & Janowska, B. (2022). Trichoderma spp. Improves Flowering, Quality, and Nutritional Status of Ornamental Plants. International Journal of Molecular Sciences, 23(24), 15662. https://doi.org/10.3390/ijms232415662

Anhar, A., Advinda, L., Putri, D. H., Atika, V., & Amimi, S. (2020, January 1). Effect of Trichoderma spp on Plant Height of Local Rice Varieties in the Early Phase of Growth. https://doi.org/10.2991/absr.k.200807.004

Ayyandurai, M., Therademani, M., Yesu Raja, I., Balakumbahan, R., Merina Prem Kumari, S., Kavitha Pushpam, A., & Revathy, N. (2024). HARNESSING TRICHODERMA SPP. FOR SUSTAINABLE PLANT DISEASE MANAGEMENT: MECHANISMS, METABOLITES AND APPLICATION STRATEGIES- A REVIEW. The Journal of Animal and Plant Sciences, 34(2), 304–317. https://doi.org/10.36899/japs.2024.2.0718

Carganilla, J., Aquino, J., Dinay-Og, J., Cumahling, G. J., & Valdez, M. (2025). Efficacy of Coconut Water as a Seed Priming Agent for Enhancing Germination and Emergence of Rice. Journal of Interdisciplinary Perspectives, 3(11), 199–205. https://doi.org/10.69569/jip.2025.663

Chagas Junior, A. F., Souza, M. C., Martins, A. L. L., Lima, C. A., Moura, D. M. D. O., Ferreira, A. L. L., Lopes, M. B., & Chagas, L. F. B. (2022). Efficiency of Trichoderma asperellum as a promoter of vegetable growth and soybean productivity. Research, Society and Development, 11(6), e50711629200. https://doi.org/10.33448/rsd-v11i6.29200

Chen, D., Hou, Q., Jia, L., & Sun, K. (2021). Combined Use of Two Trichoderma Strains to Promote Growth of Pakchoi (Brassica chinensis L.). Agronomy, 11(4), 726. https://doi.org/10.3390/agronomy11040726

Domingues Neto, F. J., Carneiro, D. C. D. S., Putti, F. F., Rodrigues, J. D., Tecchio, M. A., Leonel, S., & Silva, M. D. S. (2024). Physiological Indexes in Seed Germination and Seedling Growth of Rangpur Lime (Citrus limonia L. Osbeck) under Plant Growth Regulators. Agronomy, 14(9), 2066. https://doi.org/10.3390/agronomy14092066

Ginting, I. F., Gustian, M., Anggara, H., & Pebriyanti, P. (2025). Pengaruh Lama Perendaman Dalam Air Kelapa Terhadap Pertumbuhan Bibit Kopi (Coffea sp.) Melalui Stek Batang. Jurnal Sains Agro, 10(1), 48–54. https://doi.org/10.36355/jsa.v10i1.1755

Guchhait, P., Varma, S., Banerjee, D., Kumar, S., Halder, R., & Dahiya, A. (2024). Plant Growth Regulators and Rooting Media: A Viable Approach for Growth and Performance of Citrus. Journal of Experimental Agriculture International, 46(5), 366–378. https://doi.org/10.9734/jeai/2024/v46i52387

Gupta, N. (2020). Trichoderma as Biostimulant: Factors Responsible for Plant Growth Promotion (pp. 287–309). Springer. https://doi.org/10.1007/978-3-030-54758-5_13

Indriyanti, D. R., Rahmawati, R., Widiatningrum, T., & Purwantoyo, E. (2020). Effect of Trichoderma sp. secondary metabolite on the increase in leaf number of coconut plant. Journal of Physics: Conference Series, 1567(3), 032040. https://doi.org/10.1088/1742-6596/1567/3/032040

Jayawardena, B. M., Agampodi, V. A., & Abeywickrama, K. P. (2021). Effect of Coconut (<em>Cocos nucifera</em>) Water Extract on the Development of Adventitious Roots in <em>Polyscias fillicifolia</em> Stem Cuttings. Journal of Agricultural Sciences – Sri Lanka, 16(03), 484–490. https://doi.org/10.4038/jas.v16i03.9473

Karaca, K., & Eltem, R. (2024). Investigating Plant Growth Promoting Properties of Trichoderma Species for Sustainable Agriculture. Wiley. https://doi.org/10.22541/au.172499496.61669454/v1

Khan, R. A. A., Najeeb, S., Chen, J., Wang, R., Zhang, J., Hou, J., & Liu, T. (2023). Insights into the molecular mechanism of Trichoderma stimulating plant growth and immunity against phytopathogens. Physiologia Plantarum, 175(6), e14133. https://doi.org/10.1111/ppl.14133

Konappa, N., Krishnamurthy, S., Krishnamurthy, S., Dhamodaran, N., Arakere, U. C., Ramachandrappa, N. S., Srinivas, C., & Chowdappa, S. (2020). Beneficial Effects of Trichoderma on Plant–Pathogen Interactions: Understanding Mechanisms Underlying Genes (pp. 41–85). Springer Nature. https://doi.org/10.1007/978-3-030-54758-5_3

Kong, E. Y. Y., Biddle, J. M., Sisunandar, S., Kalaipandian, S., Bazrafshan, A., Mu, Z., & Adkins, S. W. (2025). Initiation of Direct Shoot Organogenesis in Coconut Using Immature Inflorescence. Plants, 14(20), 3123. https://doi.org/10.3390/plants14203123

Korlina, E., Hasyim, A., & Sulastrini, I. (2023). Differences in time of application of Trichoderma sp on shallot varieties on growth and disease development for seed production. IOP Conference Series: Earth and Environmental Science, 1172(1), 012039. https://doi.org/10.1088/1755-1315/1172/1/012039

Kumar, A., Rajan, R., Pandey, K., Ramprasad, R. R., Kaur, G., Vamshi, T., & Singh, T. (2024). Impact of new generation plant growth regulators on fruit crops - A Review. Horticultural Science, 51(1), 1–22. https://doi.org/10.17221/166/2022-hortsci

Madhushani, K. P. P., Subasinghe, H. M. P. A., Fonseka, R. M., & Sivananthawerl, T. (2024). Modified Canopy Architecture Enhanced Yield of Black Pepper (Piper nigrum L.) through High Absorption and Distribution of Photosynthetically Active Radiation (PAR). Tropical Agricultural Research, 35(3), 175–187. https://doi.org/10.4038/tar.v35i3.8788

Maurya, S., Ntakirutimana, R., Rana, B. D. M., & Srivastava, D. K. A. S. (2024). Trichoderma and Their Secondary Metabolites ? A Potential Approach in Plant Disease Management. BIOPESTICIDES INTERNATIONAL, 20(01), 21. https://doi.org/10.59467/bi.2024.20.21

Muleo, R., Hassan, M. I., Pellegrino, A., & Cavallaro, V. (2025). Effects of Light on Adventitious Rooting In Vitro. Agronomy, 15(11), 2597. https://doi.org/10.3390/agronomy15112597

Nikila, A., Renuka, R., Kumar, K. K., Mohanalakshmi, M., Suresh, J., & Thavaprakash, N. (2025). Advances in coconut micropropagation: prospects, constraints and way forward. Plant Science Today, 12(sp1). https://doi.org/10.14719/pst.5826

Noor, K., Khan, I. M., Nazeer, M., Jamil, M., & Anees, M. (2026). Multifunctional Traits of Trichoderma afroharzianum ISO-02 and Trichoderma brevicrassum ISO-03 Promoting Wheat Growth and Germination. Journal of Basic Microbiology, 66(4). https://doi.org/10.1002/jobm.70169

Padilla, Y. G., Gisbert-Mullor, R., López-Galarza, S., & Calatayud, Á. (2024). Grafting in Pepper to Overcome Drought, Salinity, and High Temperature. Intechopen. https://doi.org/10.5772/intechopen.114359

Qiao Er Wong, S., Haradzi, N. A., Sriskanda, D., Subramaniam, S., & Lynn Chew, B. (2024). Effects of Coconut Water and Banana Homogenate on Shoot Regeneration of Meyer Lemon (Citrus × meyeri). Pertanika Journal of Tropical Agricultural Science, 47(1), 147–158. https://doi.org/10.47836/pjtas.47.1.11

Qomariyah, M., Mudakir, I., & Puspita Sari, N. (2025). The Effect of Botanical Nutrients Enriched with Trichoderma sp. on the Growth of Cocoa Seed Radicles (Theobroma cacao L.). Pelita Perkebunan (a Coffee and Cocoa Research Journal), 41(3), 297–304. https://doi.org/10.22302/iccri.jur.pelitaperkebunan.v41i3.696

Rubayet, M. T., & Hossain, M. M. (2025). Bio-Exploration of Plant Growth-Promoting Fungus Trichoderma as a Potent Candidate for Plant Disease Management: An Overview. OnLine Journal of Biological Sciences, 25(1), 22–52. https://doi.org/10.3844/ojbsci.2025.22.52

Shifka, S. F., & Seran, T. H. (2025). Evaluation of Coconut Water as a Natural Biostimulant for Enhancing Root and Shoot Development in Acerola Cherry (Malpighia emarginata L.) Stem Cuttings. Asian Journal of Science and Applied Technology, 14(1), 16–22. https://doi.org/10.70112/ajsat-2025.14.1.4265

Sparta, A., & Emilda, D. (2020). Growth Evaluation of Banana cv. Barangan as the Effect of Trichoderma sp. and Covering Types during Acclimatization Process. Caraka Tani: Journal of Sustainable Agriculture, 35(2), 268. https://doi.org/10.20961/carakatani.v35i2.41191

Srivastava, S., Debnath, B., Manda, R. R., Maurya, S., Rana, M., & Tiwari, A. K. (2025). Trichoderma in sustainable agriculture: Advances, applications, and future prospects for biocontrol and plant growth promotion. Annals of Applied Biology, 188(1), 332–351. https://doi.org/10.1111/aab.70052

Subramaniam, S., Zainudin, N. A. I. M., Aris, A., & Hasan, Z. A. E. (2022). Role of Trichoderma in Plant Growth Promotion (pp. 257–280). Springer. https://doi.org/10.1007/978-3-030-91650-3_9

Supyanto, S., & Argus, A. (2025). Growth of Trichoderma sp. on Corn and Bran Media at the Food Crop and Horticulture Pest and Disease Observation Laboratory (PHPTPH) Lebaksari Pandaan Pasuruan. Bioconsortium: Biological Research and Education, 2(1), 21–26. https://doi.org/10.59005/bioconsortium.v2i1.722

Swelih, E. A., & Said, A. G. E. (2023). Propagation of Lime by Stem Cuttings. University of Khartoum Journal of Agricultural Sciences, 17(3). https://doi.org/10.53332/uofkjas.v17i3.1927

Triasih, U.-, & Widyaningsih, S. (2023). Test Fungi Growth Physiology of Trichoderma sp. and Gliocladium sp. from Citrus Plants. Gontor Agrotech Science Journal, 9(1), 1–10.

https://doi.org/10.21111/agrotech.v9i1.8604

Wilms, H., Bièvre, D. D., Rosiers, E., Swennen, R., Rhee, J., & Panis, B. (2022). Tissue necrosis prevention during shoot multiplication of coconut. Acta Horticulturae, 1339, 173–180. https://doi.org/10.17660/actahortic.2022.1339.23

Zhu, L., Zhao, X., Wang, C., Wang, J., Wang, P., & Tian, C. (2022). Trichoderma affects plant growth and soil ecological environment: A mini-review. Zemdirbyste-Agriculture, 109(4), 341–348. https://doi.org/10.13080/z-a.2022.109.044




DOI: https://doi.org/10.33096/agrogenesis.v2i1.1053

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ISSN: 3090-2657 Published by: Agrotechnology Study Program, Faculty of Agriculture, Universitas Muslim Indonesia, Website: fp.umi.ac.idOfficial Website: umi.ac.idEmail: agrogenesis@umi.ac.id

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