PEMANTAPAN MASA INKUBASI KALUS KEDELAI SECARA IN VITRO DALAM MEDIA MS (Murashige dan Skoog) B5 (Gamborg)

Rahmad Hidayat, Abdullah Abdullah, Aminah Muchdar

Abstract


Combination of Soybean Callus Incubation Period in vitro in MS (Murashige and Skoog) and B5 (Gamborg) Media (Supervised by Abdullah and Aminah). This study aimed to find a suitable medium for stabilizing the incubation period of soybean callus in vitro for the purposes of further selection. This research was conducted at the Laboratory of Bio-Science and Plant Reproduction Biotechnology,  Agronomy Department,  Agriculture Faculty, Hasanuddin University, Makassar, South Sulawesi. This research was done from June 2020 to October 2020. This research was conducted using a Completely Randomized Design (CRD) with two-factor treatment. The first factor was the type of medium (M1) with 2 levels of treatment. The second factor was the callus incubation period (W) with 5 levels of treatment. The data were analyzed using analysis of variance and 5% LSD. The parameters observed were callus wet weight, callus color and callus structure. The results showed that the MS subculture medium in stabilizing the incubation period of soybean callus tended to be better to callus color and callus structure. The highest wet weight of callus was in the M1W2 treatment with an average of 1.40 callus color scoring values, namely, 5.8 in the white color category and the callus structure with a scoring value of 4, namely callus which had a crumb texture (easily separated). Callus weight tends to be better on B5 medium. The incubation period for soybean callus tended to be better within 14 days, after subculturing on all types of medium (MS and B5) and subsequently decreased by increasing incubation time. The type of incubation medium did not interact with the callus incubation period.


Keywords


MS; B5; Callus; Soybean; Subculture

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